7P8K
Crystal structure of in planta processed AvrRps4 in complex with the WRKY domain of RRS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91188 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 105.607, 105.607, 66.987 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.770 - 2.650 |
| R-factor | 0.2473 |
| Rwork | 0.245 |
| R-free | 0.28380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b6x 5w3x |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.237 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.770 | 45.730 | 2.780 |
| High resolution limit [Å] | 2.650 | 8.790 | 2.650 |
| Rmerge | 0.095 | 0.031 | 1.185 |
| Rmeas | 0.097 | 0.032 | 1.211 |
| Rpim | 0.019 | 0.007 | 0.238 |
| Total number of observations | 3813 | 19413 | |
| Number of reflections | 6800 | 229 | 888 |
| <I/σ(I)> | 17.4 | 57.5 | 2.3 |
| Completeness [%] | 99.9 | 98.6 | 100 |
| Redundancy | 20.8 | 16.7 | 21.9 |
| CC(1/2) | 1.000 | 1.000 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.8M Potassium sodium tartrate tertrahydrate, 0.1M Sodium HEPES pH 7.5 |
