7P8G

Crystal structure of Mycobacterium hassiacum glucosyl-3-phosphoglycerate synthase at pH 5.5 - apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99987
Spacegroup name	I 41
Unit cell lengths	101.423, 101.423, 122.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.600 - 2.130
R-factor	0.2065
Rwork	0.205
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7p5l
RMSD bond length	0.007
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.600	2.200
High resolution limit [Å]	2.130	2.130
Rmerge	0.050	0.568
Rmeas	0.056	0.635
Rpim	0.024	0.276
Number of reflections	34136	581
<I/σ(I)>	15	1.9
Completeness [%]	98.6	98.9
Redundancy	2.5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M Bis-Tris pH 5.5, 3M NaCl