7P8C

Crystal structure of the Receiver domain of A. thaliana cytokinin receptor AtCRE1 in complex with K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	90.319, 101.426, 33.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.250 - 2.150
R-factor	0.2124
Rwork	0.209
R-free	0.28420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4euk
RMSD bond length	0.009
RMSD bond angle	1.009
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.250	2.390
High resolution limit [Å]	2.100	2.150
Rmerge	0.176	1.230
Rmeas	0.184	1.286
Rpim	0.051	0.367
Number of reflections	10946	544
<I/σ(I)>	11.4	2.6
Completeness [%]	62.2	11.4
Redundancy	12.9	11.5
CC(1/2)	0.990	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.1 M HEPES pH 7.0, 30% v/v Jeffamine M-600 pH 7.0