Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 126.279, 126.279, 115.361 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.710 - 1.450 |
| R-factor | 0.132 |
| Rwork | 0.130 |
| R-free | 0.16980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.588 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 115.360 | 115.360 | 1.470 |
| High resolution limit [Å] | 1.450 | 7.940 | 1.450 |
| Rmerge | 0.080 | 0.087 | 0.662 |
| Rmeas | 0.086 | 0.095 | 0.711 |
| Rpim | 0.032 | 0.036 | 0.259 |
| Total number of observations | 1196324 | 7605 | 59695 |
| Number of reflections | 164062 | 1171 | 7995 |
| <I/σ(I)> | 13.6 | 25.3 | 3 |
| Completeness [%] | 100.0 | 99.6 | 99.9 |
| Redundancy | 7.3 | 6.5 | 7.5 |
| CC(1/2) | 0.996 | 0.990 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.7 | 289 | 10 mM BES buffer |
