7P5U
Neuropilin-b1 in a complex with a VEGFB-derived peptide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.496, 74.350, 91.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.680 - 1.600 |
| Rwork | 0.188 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kex |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.944 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.684 | 1.670 |
| High resolution limit [Å] | 1.510 | 1.600 |
| Rmerge | 0.067 | |
| Number of reflections | 50849 | 4924 |
| <I/σ(I)> | 14 | |
| Completeness [%] | 96.7 | |
| Redundancy | 7.9 | |
| CC(1/2) | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 10 to 30% PEG 3350 + 0.2 M ammonium chloride |
