7P5L
Crystal structure of Mycobacterium hassiacum glucosyl-3-phosphoglycerate synthase at pH 7.1 - apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96112 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.098, 90.605, 95.264 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.230 - 1.220 |
| R-factor | 0.1441 |
| Rwork | 0.143 |
| R-free | 0.16380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e25 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.008 |
| Data reduction software | XDS (July 4 2012) |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.260 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.052 | 0.625 |
| Rmeas | 0.060 | 0.734 |
| Rpim | 0.028 | 0.379 |
| Number of reflections | 182244 | 17739 |
| <I/σ(I)> | 12.7 | 1.9 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 0.165 M DL-Malic Acid pH 7.1; 22% (w/v) PEG 3350 |
