7P56

Variant Surface Glycoprotein 2 (VSG2, MiTat1.2, VSG221) Bound to Calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-22
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	42.915, 87.585, 88.121
Unit cell angles	90.00, 98.14, 90.00

Refinement procedure

Resolution	61.810 - 1.735
R-factor	0.1911
Rwork	0.189
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vsg
Data reduction software	XDS (VERSION Oct 15, 2015 BUILT=20151015)
Data scaling software	XDS (VERSION Oct 15, 2015 BUILT=20151015)
Phasing software	PHENIX (PHENIX: Phaser 2.5.6)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.810	1.797
High resolution limit [Å]	1.735	1.735
Rmerge	0.077	1.565
Rmeas	0.077	1.882
Rpim	0.049	1.032
Number of reflections	65518	5809
<I/σ(I)>	10.93	0.75
Completeness [%]	97.3	86.82
Redundancy	3.3
CC(1/2)	0.998	0.307

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M K-acetate, 22% PEG8000.