7P52
GlnK1 from Methanocaldococcus jannaschii with Mg-ATP and 2-oxoglutarate at a resolution of 1.2 A
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-25 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.99187 |
Spacegroup name | H 3 2 |
格子定数 [Å] | 89.053, 89.053, 98.059 |
格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
分解能 | 44.526 - 1.200 |
R因子 | 0.1324 |
Rwork | 0.131 |
R-free | 0.15540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j9d |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 44.526 | 1.240 |
分解能 [Å] (高) | 1.200 | 1.200 |
Rmerge_l_obs | 0.057 | 1.209 |
Rmeas | 0.060 | 1.290 |
Rpim | 0.020 | 0.443 |
独立反射数 | 31673 | 1585 |
<I/σ(I)> | 17.8 | 1.7 |
完全性 [%] | 89.8 | 99.6 |
冗長性 | 9.4 | 8.3 |
CC(1/2) | 0.999 | 0.642 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | GlnK1 was crystallized at 11.2 mg/ml in 25 mM Tris/HCl pH 7.6, 10% glycerol, 2mM dithiothreitol and 500mM NaCl on 96-Well MRC 2-Drop Crystallization Plates in polystyrene (SWISSCI). GlnK1 was cocrystallized with 2 mM ATP, 2 mM 2-oxoglutarate and 2 mM MgCl2. Drop of 0.6 ul of protein sample was mixed with 0.6 ul of the crystallization solution. The reservoir contained 90 ul of the following crystallization solution: 20% PEG 3,350, 100 mM Bis-Tris propane pH 8.5 and 200 mM sodium nitrate. |