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7P52

GlnK1 from Methanocaldococcus jannaschii with Mg-ATP and 2-oxoglutarate at a resolution of 1.2 A

Experimental procedure
実験手法SINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06DA
Synchrotron siteSLS
BeamlineX06DA
Temperature [K]100
Detector technologyPIXEL
Collection date2020-02-25
DetectorDECTRIS PILATUS 2M-F
Wavelength(s)0.99187
Spacegroup nameH 3 2
格子定数 [Å]89.053, 89.053, 98.059
格子定数 [度]90.00, 90.00, 120.00
精密化法
分解能44.526 - 1.200
R因子0.1324
Rwork0.131
R-free0.15540
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2j9d
Data reduction softwareautoPROC
Data scaling softwareautoPROC
Phasing softwareMOLREP
Refinement softwarePHENIX (1.19.2_4158)
Quality characteristics
 OverallOuter shell
分解能 [Å] (低)44.5261.240
分解能 [Å] (高)1.2001.200
Rmerge_l_obs0.0571.209
Rmeas0.0601.290
Rpim0.0200.443
独立反射数316731585
<I/σ(I)>17.81.7
完全性 [%]89.899.6
冗長性9.48.3
CC(1/2)0.9990.642
結晶化条件
結晶ID方法pH温度溶液条件
1VAPOR DIFFUSION, SITTING DROP8.5291.15GlnK1 was crystallized at 11.2 mg/ml in 25 mM Tris/HCl pH 7.6, 10% glycerol, 2mM dithiothreitol and 500mM NaCl on 96-Well MRC 2-Drop Crystallization Plates in polystyrene (SWISSCI). GlnK1 was cocrystallized with 2 mM ATP, 2 mM 2-oxoglutarate and 2 mM MgCl2. Drop of 0.6 ul of protein sample was mixed with 0.6 ul of the crystallization solution. The reservoir contained 90 ul of the following crystallization solution: 20% PEG 3,350, 100 mM Bis-Tris propane pH 8.5 and 200 mM sodium nitrate.

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件を2025-04-02に公開中

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