7P4Z

Crystal structure of avidin from hen egg white in space group C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	114.424, 43.304, 62.314
Unit cell angles	90.00, 109.04, 90.00

Refinement procedure

Resolution	58.900 - 2.200
R-factor	0.2493
Rwork	0.247
R-free	0.29060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y53
RMSD bond length	0.007
RMSD bond angle	1.213
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	MOLREP
Refinement software	REFMAC (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.920	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	6.000	9.900
Rmeas	4.900
Rpim	3.000
Number of reflections	14917	695
<I/σ(I)>	15.5
Completeness [%]	99.8
Redundancy	4.6
CC(1/2)	0.990	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% w/v PEG 8000, 20% v/v ethylene glycol, 0.1 M MES/Imidazole pH 6.5, 0.4% of each Anaesthetic alkaloid (Morpheus Fusion screen, well H12)