7P4B
HLA-E*01:03 in complex with IL9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 244.562, 48.529, 153.161 |
| Unit cell angles | 90.00, 116.95, 90.00 |
Refinement procedure
| Resolution | 75.180 - 1.720 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gh1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.616 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.030 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 171397 | 8228 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.5 | |
| CC(1/2) | 1.000 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.5 | 2.2 M AS 0.1 M MES pH 5.8 |
