7P4A
Non-canonical Staphylococcus aureus pathogenicity island repression.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-11 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97936 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 100.211, 100.211, 298.688 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.478 - 2.901 |
| Rwork | 0.298 |
| R-free | 0.34280 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.321 |
| Data reduction software | iMOSFLM |
| Data scaling software | DIALS |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.760 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.049 | |
| Number of reflections | 20492 | 3215 |
| <I/σ(I)> | 14.2 | 2.4 |
| Completeness [%] | 99.7 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.846 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.4M Ammonium phosphate, 25% PEG200 |
