7P45

Structure of CgGBE in P212121 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	79.580, 89.230, 112.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.450 - 2.090
R-factor	0.1926
Rwork	0.191
R-free	0.23330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bzy
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.450	2.220
High resolution limit [Å]	2.090	2.090
Rmeas	0.117
Number of reflections	43538	6892
<I/σ(I)>	14.7	1.66
Completeness [%]	99.9	99.4
Redundancy	13.5	13.9
CC(1/2)	0.999	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	292	0.1M MES pH 6.5, 25% (w/v) PEG 4000