7P44

Structure of CgGBE in P21212 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 2
Unit cell lengths	210.026, 88.615, 89.723
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.850 - 2.400
R-factor	0.2126
Rwork	0.211
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bzy
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.850	2.500
High resolution limit [Å]	2.400	2.400
Rmeas	0.174	0.854
Number of reflections	65906	7507
<I/σ(I)>	6.62	1.58
Completeness [%]	99.4
Redundancy	4.9
CC(1/2)	0.994	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.6	292	0.1M trisodium citrate pH 5.6, 10% PEG 4000, 10% isopropanol