7P43

Structure of CgGBE in complex with maltotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 2
Unit cell lengths	88.720, 210.700, 90.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.050 - 1.930
R-factor	0.1932
Rwork	0.192
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bzy
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.050	2.040
High resolution limit [Å]	1.930	1.930
Rmeas	0.090
Number of reflections	128265	20066
<I/σ(I)>	13.38	1.76
Completeness [%]	99.6	97.6
Redundancy	6.5	6.4
CC(1/2)	0.990	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.6	292	0.1M trisodium citrate pH 5.6, 10% PEG 4000, 10% isopropanol