7P3T

Transaminase of gamma-proteobacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	86.810, 144.580, 151.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.933 - 1.600
R-factor	0.1695
Rwork	0.169
R-free	0.18470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cm0
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.933	45.933	1.700
High resolution limit [Å]	1.600	12.000	1.600
Rmerge	0.036	0.016	0.879
Rmeas	0.038	0.017	0.968
Total number of observations	2275575
Number of reflections	250022	674	40538
<I/σ(I)>	32.66	108.13	2.04
Completeness [%]	99.7	94.4	98.1
Redundancy	9.101	8.248	5.532
CC(1/2)	1.000	1.000	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	20% PEG 3350 0.2 M NaNO3