7P1Z

Novel GH12 endogluconase from Aspergillus cervinus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.8
Spacegroup name	P 2 21 21
Unit cell lengths	46.094, 118.364, 125.702
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.930 - 2.170
Rwork	0.183
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gm3
RMSD bond length	0.008
RMSD bond angle	1.413
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	125.700	125.700	2.240
High resolution limit [Å]	2.170	8.950	2.170
Rmerge	0.152	0.046	0.715
Rmeas	0.180	0.054	0.862
Rpim	0.095	0.029	0.475
Number of reflections	37115	626	3144
<I/σ(I)>	7.8
Completeness [%]	99.6
Redundancy	6.5	5.5	6.1
CC(1/2)	0.995	0.989	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		288	Peg 6000 18,5% 0.2M CaCl2 0.1M NaAcetate pH 5.0