7P1B
Apo structure of KDNase from Aspergillus Terrerus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2018-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 61.441, 67.280, 93.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.810 - 1.450 |
| R-factor | 0.2176 |
| Rwork | 0.216 |
| R-free | 0.25570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xcy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.329 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.810 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 69104 | 5046 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.990 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 6K, 0.1M MES, pH6, 0.2M Calcium Chloride |
