7P0S

ORF virus encoded Bcl-2 homolog ORFV125 in complex with Puma BH3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	49.033, 69.564, 92.117
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.283 - 2.503
R-factor	0.246848326679
Rwork	0.245
R-free	0.28583
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7adt
RMSD bond length	0.002
RMSD bond angle	0.478
Data reduction software	DIALS
Data scaling software	Aimless (7.0.078)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575_000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.283	2.600
High resolution limit [Å]	2.503	2.503
Rmerge	0.096	1.350
Number of reflections	11059	1219
<I/σ(I)>	8.91
Completeness [%]	97.0	98.4
Redundancy	4.1
CC(1/2)	0.999	0.296

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Magnesium acetate, 25% PEG 3350