7P0O
mitoNEET bound to M1 molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-30 |
| Detector | DECTRIS PILATUS3 S 1M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.152, 49.962, 58.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.110 - 1.650 |
| R-factor | 0.17435 |
| Rwork | 0.171 |
| R-free | 0.23645 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ew0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.190 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.150 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.105 | 0.019 |
| Number of reflections | 15754 | 1151 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 32% PEG-3000, 100 mM Tris-HCl (pH 8.0) and100mM NaCl. |
