7P0L
Crystal structure of S.pombe Mdb1 BRCT domains in complex with a H2A phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.380, 42.050, 96.290 |
| Unit cell angles | 90.00, 91.87, 90.00 |
Refinement procedure
| Resolution | 48.120 - 1.970 |
| R-factor | 0.2098 |
| Rwork | 0.208 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7p0j |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.819 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.120 | 2.020 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rpim | 0.044 | 0.383 |
| Number of reflections | 23405 | 1720 |
| <I/σ(I)> | 1.087 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200mM Magnesium Chloride 100mM TRIS pH 8.0 30% (w/v) PEG 4000 |
