7P0J
Crystal structure of S.pombe Mdb1 BRCT domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97957 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.180, 44.120, 49.860 |
| Unit cell angles | 90.00, 92.37, 90.00 |
Refinement procedure
| Resolution | 49.820 - 1.480 |
| R-factor | 0.1715 |
| Rwork | 0.170 |
| R-free | 0.19650 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.092 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.820 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rpim | 0.036 | 0.519 |
| Number of reflections | 41143 | 3071 |
| <I/σ(I)> | 1.306 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M sodium citrate 0.1 M Bis Tris propane 7.5 20 % w/v PEG 3350 |
