7P0C

URATE OXIDASE WITH 8-AZAXANTHINE UNDER 210 MPA PRESSURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE CRISTAL
Synchrotron site	SOLEIL
Beamline	CRISTAL
Temperature [K]	293
Detector technology	CCD
Collection date	2019-07-28
Detector	RAYONIX SX-165mm
Wavelength(s)	0.58183
Spacegroup name	I 2 2 2
Unit cell lengths	79.530, 95.630, 104.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.150
R-factor	0.1924
Rwork	0.189
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 4OP9
RMSD bond length	0.014
RMSD bond angle	1.659
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.588	2.270
High resolution limit [Å]	2.150	2.150
Rmerge		0.701
Number of reflections	21075	3071
<I/σ(I)>	5.6	1
Completeness [%]	96.0	97.1
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	298	20 MG/ML URATE OXIDASE, 8-AZAXANTHINE CONCENTRATION IN EXCESS, 50 MM TRIS/ACETATE, 8% PEG 4000