7P07

Structure of the maltase BaAG2 from Blastobotrys adeninivorans in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979965
Spacegroup name	P 1 21 1
Unit cell lengths	66.880, 77.730, 121.110
Unit cell angles	90.00, 92.90, 90.00

Refinement procedure

Resolution	47.730 - 2.130
R-factor	0.2154
Rwork	0.215
R-free	0.23180
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7p01
RMSD bond length	0.017
RMSD bond angle	1.890
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.730	2.190
High resolution limit [Å]	2.130	2.130
Number of reflections	134791	10026
<I/σ(I)>	8.61	1.19
Completeness [%]	98.9	99.1
Redundancy	3.55	3.58
CC(1/2)	0.998	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1 M MES (pH 5.5) 12% polyvinylpyrrolidone