7OZ6
Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MC)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.289, 87.283, 93.925 |
| Unit cell angles | 90.00, 130.15, 90.00 |
Refinement procedure
| Resolution | 46.732 - 1.757 |
| Rwork | 0.183 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7os3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.652 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.732 | 1.860 |
| High resolution limit [Å] | 1.757 | 1.760 |
| Rmerge | 0.556 | |
| Number of reflections | 78980 | 12374 |
| <I/σ(I)> | 22.04 | 2.69 |
| Completeness [%] | 98.3 | 95.7 |
| Redundancy | 6.74 | 6.59 |
| CC(1/2) | 1.000 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 25% PEG3350, 0.2 M Li2SO4, 0.01% (w/v) heptane-1,2,3-triol, 0.1 M Tris pH 8.0 |
