7OZ6

Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MC)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	130.289, 87.283, 93.925
Unit cell angles	90.00, 130.15, 90.00

Refinement procedure

Resolution	46.732 - 1.757
Rwork	0.183
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7os3
RMSD bond length	0.015
RMSD bond angle	1.652
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.732	1.860
High resolution limit [Å]	1.757	1.760
Rmerge		0.556
Number of reflections	78980	12374
<I/σ(I)>	22.04	2.69
Completeness [%]	98.3	95.7
Redundancy	6.74	6.59
CC(1/2)	1.000	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	25% PEG3350, 0.2 M Li2SO4, 0.01% (w/v) heptane-1,2,3-triol, 0.1 M Tris pH 8.0