7OYU
E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D Y87S F88Y in complex with spermidine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-11 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.136, 78.606, 114.831 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.360 - 1.950 |
R-factor | 0.2029 |
Rwork | 0.201 |
R-free | 0.24180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a99 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.679 |
Data reduction software | XDS (20190315) |
Data scaling software | XSCALE (20190315) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.360 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.206 | 2.231 |
Rmeas | 0.227 | 2.451 |
Rpim | 0.093 | 0.996 |
Number of reflections | 25021 | 14023 |
<I/σ(I)> | 6.44 | 0.79 |
Completeness [%] | 98.9 | 99.2 |
Redundancy | 5.5 | 5.7 |
CC(1/2) | 0.994 | 0.262 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 M Sodium acetate pH 4.6, 0.2 M Ammonium acetate, 30% PEG 4000 |