7OY0
Structure of human Spermine Oxidase in complex with a highly selective allosteric inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000040 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 193.755, 193.755, 44.332 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 167.800 - 2.090 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | none |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.372 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 167.800 | 2.400 |
| High resolution limit [Å] | 2.090 | 2.095 |
| Rmerge | 0.068 | 1.407 |
| Number of reflections | 27031 | 1354 |
| <I/σ(I)> | 18.9 | 2 |
| Completeness [%] | 48.2 | 70 |
| Redundancy | 13 | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.01 M Na Acet, 0.06 M NaF |
