7OX9

Target-bound SpCas9 complex with AAVS1 all-RNA guide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-22
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	178.080, 68.205, 187.771
Unit cell angles	90.00, 111.14, 90.00

Refinement procedure

Resolution	48.160 - 2.450
R-factor	0.1968
Rwork	0.195
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fq5
RMSD bond length	0.003
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.160	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.140	1.606
Number of reflections	77217	7600
<I/σ(I)>	10.2	1.04
Completeness [%]	99.2	98.69
Redundancy	6.7	6.4
CC(1/2)	0.997	0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	0.1 M Tris-acetate pH 8.5, 0.3-0.5 M KSCN, 17-19% PEG3350