7OWX

Structure of coiled-coil tetramer from SARS-CoV-2 spike stalk region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 21 21 21
Unit cell lengths	45.140, 45.540, 47.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.850 - 1.929
R-factor	0.1779
Rwork	0.172
R-free	0.23290
Structure solution method	AB INITIO PHASING
RMSD bond length	0.006
RMSD bond angle	0.730
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Arcimboldo
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.850	2.050
High resolution limit [Å]	1.929	1.929
Rmeas	0.150	1.430
Number of reflections	7780	729
<I/σ(I)>	11.5
Completeness [%]	99.3	93.5
Redundancy	10.8	18.1
CC(1/2)	0.998	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	0.002 M zinc chloride, 0.1 M Tris pH 8, 20 % w/v PEG 6000