7OWP

HsNMT1 in complex with both MyrCoA and ACE-G-(L-ORN)SFSKPR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98400
Spacegroup name	P 21 21 2
Unit cell lengths	79.324, 178.199, 58.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.550 - 1.810
R-factor	0.157
Rwork	0.155
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o9t
RMSD bond length	0.006
RMSD bond angle	0.862
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.720	1.850
High resolution limit [Å]	1.810	1.810
Rpim	0.037	0.027
Number of reflections	143724	4375
<I/σ(I)>	16.9	4.8
Completeness [%]	99.1	98.2
Redundancy	15
CC(1/2)	0.999	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	18% PEG8K, 100mM Sodium Citrate pH 5.6, 100mM MgCl2, 100mM NaCl