7OWF
KlenTaq DNA polymerase in a ternary complex with primer/template and the fluorescent nucleotide analog BFdUTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000089622351307 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.175, 110.175, 91.296 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.710 - 1.910 |
| R-factor | 0.1977 |
| Rwork | 0.195 |
| R-free | 0.24120 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19_4085) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.710 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 96475 | 15157 |
| <I/σ(I)> | 7.45 | 0.46 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 7.5 | |
| CC(1/2) | 0.997 | 0.247 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291.15 | 16% PEG 6000, 0.1M Tris pH 8.0, 0.2M magnesium formate |
