7OWD

Structure of CYLD CAP-Gly3 (467-552) bound to Ub; tetragonal space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	210
Detector technology	PIXEL
Collection date	2018-04-15
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.96863
Spacegroup name	P 41 21 2
Unit cell lengths	42.931, 42.931, 171.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.640 - 1.710
R-factor	0.2151
Rwork	0.214
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 1ixd
RMSD bond length	0.017
RMSD bond angle	1.724
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.640	1.740
High resolution limit [Å]	1.710	1.710
Number of reflections	18356	933
<I/σ(I)>	15	1.9
Completeness [%]	100.0
Redundancy	6
CC(1/2)	0.998	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	291	40 (v/v) PEG 300, 100 mM phosphate/citrate pH 4.2