7OWC

Structure of CYLD CAP-Gly3 (467-565) bound to Ub; orthorhobic space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.96863
Spacegroup name	P 21 21 21
Unit cell lengths	63.927, 64.486, 75.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.150 - 1.850
R-factor	0.2076
Rwork	0.206
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 1ixd
RMSD bond length	0.006
RMSD bond angle	0.801
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.490	1.890
High resolution limit [Å]	1.850	1.850
Number of reflections	27492	1655
<I/σ(I)>	14	2.1
Completeness [%]	100.0
Redundancy	6.3
CC(1/2)	0.999	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	291	35 % (w/v) PEG 1,000, 50 mM HEPES pH 7.3