7OV9
Heterodimeric tRNA-Guanine Transglycosylase from mouse, apo-structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.918400 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 100.257, 100.257, 202.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.660 - 1.900 |
| R-factor | 0.1767 |
| Rwork | 0.175 |
| R-free | 0.19930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h62 6fv5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.781 |
| Data reduction software | XDS (1.02) |
| Data scaling software | XDS (1.02) |
| Phasing software | PHASER (7.0.047) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 82034 | 13000 |
| <I/σ(I)> | 24.55 | |
| Completeness [%] | 99.9 | |
| Redundancy | 14.57 | |
| CC(1/2) | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 100 mM Citrate pH 6.5, 700 mM Ammonium sulfate, 1 M Lithium sulfate |
