7OUD
Crystal structure of a ternary complex of the flavoprotein monooxygenase GrhO5 with FAD and collinone
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.666, 103.747, 69.005 |
| Unit cell angles | 90.00, 104.59, 90.00 |
Refinement procedure
| Resolution | 44.190 - 2.300 |
| Rwork | 0.225 |
| R-free | 0.26290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RubL |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.543 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.190 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.200 | 0.490 |
| Number of reflections | 26914 | 3911 |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.920 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 20 % PEG 3350, 200mM di-sodium malonate |
