7OU1
Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MP2)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.923, 91.308, 114.164 |
| Unit cell angles | 90.00, 97.11, 90.00 |
Refinement procedure
| Resolution | 45.650 - 1.650 |
| Rwork | 0.190 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7os3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.645 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.650 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.134 | 0.622 |
| Number of reflections | 189888 | 30255 |
| <I/σ(I)> | 10 | 2.2 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 4 | 4.1 |
| CC(1/2) | 0.995 | 0.795 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 2% PEG8000, 20% PEG400, 5 mM Mg(CH3COO)2, 6 mM 3.0 mM sucrose monolaureate |
