7OSA

Pre-translocation complex of 80 S.cerevisiae ribosome with eEF2 and ligands

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	227.110, 309.350, 527.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	154.680 - 3.000
R-factor	0.196
Rwork	0.196
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v88
RMSD bond length	0.008
RMSD bond angle	1.307
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	154.680	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	734615	72990
<I/σ(I)>	20.12	0.95
Completeness [%]	100.0
Redundancy	206
CC(1/2)	0.990	0.230

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG10K, KSCN, TRIS-HAC