7OS5

Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (orthorhombic form OP)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	78.231, 91.142, 106.017
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.110 - 1.293
Rwork	0.155
R-free	0.19430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7os3
RMSD bond length	0.012
RMSD bond angle	1.626
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.110	1.400
High resolution limit [Å]	1.290	1.290
Rmerge	0.170	2.008
Rmeas	0.179	2.113
Rpim	0.050	0.639
Number of reflections	131038	6526
<I/σ(I)>	10.4	1.5
Completeness [%]	95.4	64.7
Redundancy	12.7	10.4
CC(1/2)	0.996	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	25% PEG3350, 0.2 M Li2SO4, 0.01% (w/v) heptane-1,2,3-triol, 0.1 M Tris pH 8.0