7OR6

The crystal structure of the domain-swapped dimer of onconase

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	28.290, 60.050, 101.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.719 - 2.120
Rwork	0.185
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1onc
RMSD bond length	0.009
RMSD bond angle	1.564
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.720	2.150
High resolution limit [Å]	2.120	2.120
Rmeas	0.273
Number of reflections	10440	10440
<I/σ(I)>	9.1	2.2
Completeness [%]	99.9	100
Redundancy	12.2
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350 0.2 M sodium citrate tribasic