7OQU
Ternary complex of 14-3-3 sigma, Amot-p130 phosphopeptide, and WQ180
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.611, 111.835, 62.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.450 - 1.400 |
| R-factor | 0.1718 |
| Rwork | 0.171 |
| R-free | 0.18240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jc3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.933 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.450 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.107 | 1.672 |
| Number of reflections | 57063 | 2810 |
| <I/σ(I)> | 12.1 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.9 | 13 |
| CC(1/2) | 0.998 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.095 M Hepes pH7.5, 26%PEG 400, 0.19 M CaCl2, and 5 % Glycerol |
