7OOK

Bacteriophage PRD1 Major Capsid Protein P3 in complex with CPZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	116.750, 122.750, 126.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.830 - 2.230
R-factor	0.1776
Rwork	0.176
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hx6
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.500	2.092
High resolution limit [Å]	2.020	2.020
Number of reflections	120623	11888
<I/σ(I)>	14.03
Completeness [%]	100.0	100
Redundancy	13.1	13.3
CC(1/2)	0.999	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	please see: PMID: 10499799