7OO9
Structure of Chloroflexus islandicus LOV domain C85A variant (CisFbFP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.606, 62.541, 64.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.780 - 1.200 |
| R-factor | 0.1592 |
| Rwork | 0.158 |
| R-free | 0.18460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rhf |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.052 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.160 | 64.160 | 1.220 |
| High resolution limit [Å] | 1.200 | 6.570 | 1.200 |
| Rmerge | 0.069 | 0.051 | 0.931 |
| Rmeas | 0.075 | 0.058 | 1.026 |
| Rpim | 0.031 | 0.025 | 0.426 |
| Total number of observations | 474237 | 3043 | 21433 |
| Number of reflections | 77945 | 572 | 3790 |
| <I/σ(I)> | 12.3 | 24.8 | 1.8 |
| Completeness [%] | 99.8 | 99.8 | 99.4 |
| Redundancy | 6.1 | 5.3 | 5.7 |
| CC(1/2) | 0.997 | 0.994 | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.04 M KH2PO4, 16% w/v PEG 8000, 20% v/v glycerol |
