7ON4
SaFtsZ complexed with GDP (co-crystalization with 1mM EDTA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.245, 51.648, 87.940 |
| Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
| Resolution | 40.830 - 1.790 |
| R-factor | 0.1595 |
| Rwork | 0.157 |
| R-free | 0.20270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rvn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.139 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.830 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 27563 | 2590 |
| <I/σ(I)> | 11.33 | 3.18 |
| Completeness [%] | 95.6 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.996 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.5, and 26% polyethylene glycol 5000 monomethyl ether, 1 mM EDTA |
