7OML

Bacillus subtilis phosphoglucomutase GlmM (metal bound)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-17
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.99
Spacegroup name	P 32 2 1
Unit cell lengths	134.418, 134.418, 69.012
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.149 - 2.900
R-factor	0.1921
Rwork	0.190
R-free	0.23420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Bacillus anthracis GlmM 3PDK
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.150	3.080
High resolution limit [Å]	2.900	2.900
Rpim	0.092	1.460
Number of reflections	15955	2503
<I/σ(I)>	12.6
Completeness [%]	98.8	97.3
Redundancy	20.2
CC(1/2)	0.990	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.05 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 30% v/v Polyethylene glycol monomethyl ether 550 buffer