7OM9

Thosea asigna virus RdRP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97934
Spacegroup name	C 1 2 1
Unit cell lengths	215.152, 74.438, 96.562
Unit cell angles	90.00, 104.65, 90.00

Refinement procedure

Resolution	48.520 - 3.000
R-factor	0.2592
Rwork	0.258
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xhi
RMSD bond length	0.004
RMSD bond angle	0.728
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.520	3.107
High resolution limit [Å]	3.000	3.000
Rmerge		1.217
Rmeas	0.355
Rpim	0.155	0.573
Number of reflections	29359	2956
<I/σ(I)>	10.52	1.45
Completeness [%]	98.2	99.29
Redundancy	4.8
CC(1/2)	0.941	0.514

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium acetate pH4.6, 0.25M (NH4)SO4, 26% PEG4000