7OM5
Anti-EGFR nanobody EgB4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9688 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.539, 71.576, 53.204 |
| Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
| Resolution | 42.690 - 1.480 |
| R-factor | 0.1786 |
| Rwork | 0.177 |
| R-free | 0.20510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4krn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.683 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.690 | 1.500 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.125 | 1.681 |
| Rmeas | 0.147 | 1.981 |
| Rpim | 0.078 | 1.037 |
| Number of reflections | 47214 | 2189 |
| <I/σ(I)> | 5.4 | 1.1 |
| Completeness [%] | 98.0 | 93 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.990 | 0.277 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 M zinc acetate and 20 % w/v PEG3350 |
