7OM3

Crystal structure of KOD DNA Polymerase in a binary complex with Hypoxanthine containing template

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000089622351307
Spacegroup name	P 21 21 2
Unit cell lengths	113.356, 142.936, 65.582
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.450 - 1.920
R-factor	0.1841
Rwork	0.183
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k8z
RMSD bond length	0.012
RMSD bond angle	1.214
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4085)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.320	2.030
High resolution limit [Å]	1.910	1.910
Rmeas	0.148
Number of reflections	147921	16579
<I/σ(I)>	7.39
Completeness [%]	93.3	64.8
Redundancy	4.6	2.2
CC(1/2)	0.996	0.185

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291.15	Morpheus E7: 0.12 M ethylene glycols (0.3 M diethylene glycol, 0.3 M triethylene glycol, 0.3 M tetraethylene glycol, 0.3 M pentaethylene glycol), 0.1 M sodium HEPES/MOPS pH 7.5, 50 % (v/v) of a mixture of glycerol (40 % v/v) and PEG 4000 (20 % w/v).