7OLN

Structure of the N-terminal domain of BC2L-C lectin (1-131) in complex with Lewis y antigen

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980
Spacegroup name	H 3 2
Unit cell lengths	42.887, 42.887, 310.001
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.880 - 1.920
Rwork	0.152
R-free	0.19340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wq4
RMSD bond length	0.015
RMSD bond angle	1.973
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.880	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.073	0.399
Rmeas	0.077	0.424
Rpim	0.026	0.143
Number of reflections	8958	601
<I/σ(I)>	23.8	7.3
Completeness [%]	100.0	100
Redundancy	15.6
CC(1/2)	0.999	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	sodium citrate 1.2M