7OLH

Bacillus subtilis Complex structure 1 of diadenylate cyclase CdaA cytoplasmic domain (CdaACD) and the phosphoglucomutase GlmM short variant (GlmMF369)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.99
Spacegroup name	P 1 21 1
Unit cell lengths	62.205, 227.561, 151.604
Unit cell angles	90.00, 99.66, 90.00

Refinement procedure

Resolution	61.320 - 3.650
R-factor	0.2428
Rwork	0.242
R-free	0.26150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Dimers of B. subtilis GlmMF369 and CdaACD
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.323	3.780
High resolution limit [Å]	3.650	3.650
Rpim	0.019	0.153
Number of reflections	45925	4441
<I/σ(I)>	5.5	1.2
Completeness [%]	99.6
Redundancy	6.9
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.12 M alcohols, 0.1 M buffer system (Imidazole; MES, pH 6.5) and 30% GOL_P4K (60% glycerol, PEG 4K)