7OL4
Mouse contactin-1 neurofascin-155 immunoglobulin domains adhesion complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-13 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 146.773, 151.775, 162.340 |
| Unit cell angles | 90.00, 111.78, 90.00 |
Refinement procedure
| Resolution | 75.380 - 4.800 |
| R-factor | 0.2901 |
| Rwork | 0.288 |
| R-free | 0.32580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ok5 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.000 | 5.352 |
| High resolution limit [Å] | 4.795 | 4.795 |
| Number of reflections | 15712 | 786 |
| <I/σ(I)> | 4.5 | 1.3 |
| Completeness [%] | 88.5 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.993 | 0.399 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277.15 | 2% v/v TacsimateTM pH 5.0, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, and 16% w/v PEG 3,350 |
