7OL3

Human ATL1 N417ins (catalytic core)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	110
Detector technology	PIXEL
Collection date	2020-03-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96860
Spacegroup name	P 21 21 21
Unit cell lengths	49.090, 115.003, 184.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.430 - 1.900
R-factor	0.1745
Rwork	0.173
R-free	0.22010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4idq
RMSD bond length	0.007
RMSD bond angle	0.864
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.430	1.968
High resolution limit [Å]	1.900	1.900
Number of reflections	82941	8042
<I/σ(I)>	8.79
Completeness [%]	99.8
Redundancy	7.3
CC(1/2)	0.992	0.335

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris-HCl pH 8.1, 2% Tacsimate pH 8, 18% PEG 3,350, 25% Glycerol (cryo-protection)